Consulta: creatorFacets:"Santos-López,Gerardo"
Registros recuperados: 4
Data/hora: 17/05/2024 17:26:42

Estandarización de un método de detección molecular del virus influenza (H5N1) de alta patogenicidad
Provedor de dados: 38
Autores: Montalvo-Corral,Maricela; Reséndiz,Mónica; Santos-López,Gerardo; Vallejo-Ruiz,Verónica; Reyes-Leyva,Julio; Hernández,Jesús.
El virus de la influenza aviar H5N1 de alta patogenicidad mantiene el alerta mundial debido a su potencial zoonótico y pandémico. Surge entonces la necesidad de contar con herramientas para la detección temprana y de esta forma reducir el impacto potencial a la salud humana y animal. En este estudio se estandarizó un método de detección molecular de los genes de la matriz (M), hemaglutinina (H5) y neuraminidasa (N1) del virus de la influenza aviar H5N1 de alta patogenicidad de linaje asiático, mediante transcripción-reversa y reacción en cadena de la polimerasa en tiempo real (RRT-PCR). A partir de un ARN viral de referencia cepa A/Vietnam/1203/2004 (H5N1) se construyeron controles positivos mediante clonación de productos de PCR. Los estándares de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Influenza aviar; Detección; Transcripción reversa-reacción en cadena de la polimerasa en tiempo real.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572009000100009

Human papillomavirus infection in women from Tlaxcala, Mexico
Provedor de dados: 58
Autores: Velázquez-Márquez,Noé; Jiménez-Aranda,Lucio Jaime; Sánchez-Alonso,Patricia; Santos-López,Gerardo; Reyes-Leyva,Julio; Vallejo-Ruiz,Verónica.
Cervical cancer is an important health problem in women living in developing countries. Infection with some genotypes of human papillomavirus (HPV) is the most important risk factor associated with cervical cancer. Little information exists about HPV genotype distribution in rural and suburban regions of Mexico. Thus, we determined the prevalence of HPV genotypes in women from Tlaxcala, one of the poorest states in central Mexico, and we evaluated age infection prevalence and risk factors associated with cervical neoplasm. A cross-sectional study was conducted in 236 women seeking gynecological care at the Mexican Institute for Social Security in Tlaxcala, Mexico. Cervical scrapings were diagnosed as normal, low-grade, and high-grade squamous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiology; Human papillomavirus; Mexico; PCR; Squamous intraepithelial lesions.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300027

Structure-function analysis of two variants of mumps virus hemagglutinin-neuraminidase protein
Provedor de dados: 55
Autores: Santos-López,Gerardo; Scior,Thomas; Borraz-Argüello,María del Tránsito; Vallejo-Ruiz,Verónica; Herrera-Camacho,Irma; Tapia-Ramírez,José; Reyes-Leyva,Julio.
A point mutation from guanine (G) to adenine (A) at nucleotide position 1081 in the hemagglutinin-neuraminidase (HN) gene has been associated with neurovirulence of Urabe AM9 mumps virus vaccine. This mutation corresponds to a glutamic acid (E) to lysine (K) change at position 335 in the HN glycoprotein. We have experimentally demonstrated that two variants of Urabe AM9 strain (HN-A1081 and HN-G1081) differ in neurotropism, sialic acidbinding affinity and neuraminidase activity. In the present study, we performed a structure-function analysis of that amino acid substitution; the structures of HN protein of both Urabe AM9 strain variants were predicted. Based on our analysis, the E/K mutation changes the protein surface properties and to a lesser extent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemagglutinin-neuraminidasa; Mumps virus; Paramyxovirus; 3D structure prediction; Sialic acid.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100007

Transcription of interferon stimulated genes in response to porcine rubulavirus infection in vitro
Provedor de dados: 58
Autores: Flores-Ocelotl,María del Rosario; Rosas-Murrieta,Nora Hilda; Vallejo-Ruiz,Verónica; Reyes-Leyva,Julio; Herrera-Camacho,Irma; Santos-López,Gerardo.
Porcine rubulavirus (PoRV) is an emerging virus causing meningo-encephalitis and reproductive failures in pigs. Little is known about the pathogenesis and immune evasion of this virus; therefore research on the mechanisms underlying tissue damage during infection is essential. To explore these mechanisms, the effect of PoRV on the transcription of interferon (IFN) pathway members was analyzed in vitro by semi-quantitative RT-PCR. Ten TCID50 of PoRV stimulated transcription of IFNα, IFNβ, STAT1, STAT2, p48 and OAS genes in neuroblastoma cells, whereas infection with 100 TCID50 did not stimulate transcription levels more than non-infected cells. When the cells were primed with IFNα, infection with 1 TCDI50 of PoRV sufficed to stimulate the transcription of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Porcine rubulavirus; Interferon; STAT; LPMV; Gene expression.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300041